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recombinant human il 2  (InvivoGen)


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    Structured Review

    InvivoGen recombinant human il 2
    Recombinant Human Il 2, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+il-6/Recombinant+human+IL-6/pm42018652-207-22-19
    Average 94 stars, based on 39 article reviews
    recombinant human il 2 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Isolation:

    Article Title: NLRP3 inhibition by VTX3232 tempers inflammation resulting in reduced body weight, hyperglycemia, and hepatic steatosis in obese male mice
    Article Snippet: (final concentration (f.c.)), and 6.25 ng/mL selenous acid (f.c.), and 25 % HHCM with 5 % heat-inactivated human serum, supplemented or not with palmitic acid (Sanbio, #29558-5, 62.5 μM f.c.), IL-1β (Invivogen #rcyc-hil1b, 10 or 20 ng/mL f.c.), IL-6 (Invivogen, rcyec-hil6, 5 ng/mL f.c.) or 10 ng/mL IL-1β + 5 ng/mL IL-6 (f.c.), or 25 % conditioned medium. On days 3 and 5, half of the medium in each

    Recombinant:

    Article Title: NLRP3 inhibition by VTX3232 tempers inflammation resulting in reduced body weight, hyperglycemia, and hepatic steatosis in obese male mice
    Article Snippet: (final concentration (f.c.)), and 6.25 ng/mL selenous acid (f.c.), and 25 % HHCM with 5 % heat-inactivated human serum, supplemented or not with palmitic acid (Sanbio, #29558-5, 62.5 μM f.c.), IL-1β (Invivogen #rcyc-hil1b, 10 or 20 ng/mL f.c.), IL-6 (Invivogen, rcyec-hil6, 5 ng/mL f.c.) or 10 ng/mL IL-1β + 5 ng/mL IL-6 (f.c.), or 25 % conditioned medium. On days 3 and 5, half of the medium in each

    Activity Assay:

    Article Title: NLRP3 inhibition by VTX3232 tempers inflammation resulting in reduced body weight, hyperglycemia, and hepatic steatosis in obese male mice
    Article Snippet: (final concentration (f.c.)), and 6.25 ng/mL selenous acid (f.c.), and 25 % HHCM with 5 % heat-inactivated human serum, supplemented or not with palmitic acid (Sanbio, #29558-5, 62.5 μM f.c.), IL-1β (Invivogen #rcyc-hil1b, 10 or 20 ng/mL f.c.), IL-6 (Invivogen, rcyec-hil6, 5 ng/mL f.c.) or 10 ng/mL IL-1β + 5 ng/mL IL-6 (f.c.), or 25 % conditioned medium. On days 3 and 5, half of the medium in each

    Enzymatic Assay:

    Article Title: NLRP3 inhibition by VTX3232 tempers inflammation resulting in reduced body weight, hyperglycemia, and hepatic steatosis in obese male mice
    Article Snippet: (final concentration (f.c.)), and 6.25 ng/mL selenous acid (f.c.), and 25 % HHCM with 5 % heat-inactivated human serum, supplemented or not with palmitic acid (Sanbio, #29558-5, 62.5 μM f.c.), IL-1β (Invivogen #rcyc-hil1b, 10 or 20 ng/mL f.c.), IL-6 (Invivogen, rcyec-hil6, 5 ng/mL f.c.) or 10 ng/mL IL-1β + 5 ng/mL IL-6 (f.c.), or 25 % conditioned medium. On days 3 and 5, half of the medium in each



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    Image Search Results


    Network pharmacology and molecular docking analysis of loganin-associated targets in UC. ( A ) Venn diagram showing the overlap between loganin-related targets and UC-associated genes. ( B ) Protein-protein interaction (PPI) network of the overlapping targets, highlighting key hub genes involved in loganin’s potential mechanisms, including TNF, EGFR, IL6, STAT3, and BCL2. ( C ) Molecular docking of loganin with IL-6. ( D ) Molecular docking of loganin with STAT3.

    Journal: Scientific Reports

    Article Title: Loganin alleviates oxidative stress-induced apoptosis by modulating mitochondrial function and STAT3 signaling in DSS-induced colitis and H 2 O 2 -injured Caco-2 cells

    doi: 10.1038/s41598-026-55447-3

    Figure Lengend Snippet: Network pharmacology and molecular docking analysis of loganin-associated targets in UC. ( A ) Venn diagram showing the overlap between loganin-related targets and UC-associated genes. ( B ) Protein-protein interaction (PPI) network of the overlapping targets, highlighting key hub genes involved in loganin’s potential mechanisms, including TNF, EGFR, IL6, STAT3, and BCL2. ( C ) Molecular docking of loganin with IL-6. ( D ) Molecular docking of loganin with STAT3.

    Article Snippet: To establish the oxidative stress model, Caco-2 cells were exposed to H 2 O 2 (100 μM) for 12 h. For interleukin-6 (IL-6) supplementation experiments, recombinant human IL-6 (MedChemExpress, HY-P7044) was added at 10 ng/mL for 24 h in the H 2 O 2 + loganin group before subsequent analyses, with reference to previous studies using IL-6 stimulation in Caco-2 cells .

    Techniques:

    Loganin inhibits apoptosis through the IL-6/STAT3/BCL2 signaling pathway in H 2 O 2 -induced epithelial cell injury. ( A ) Western blot analysis of apoptosis-related proteins Bcl-2, BAX, pro-caspase-3, and cleaved caspase-3. ( B – D ) Quantification of BAX, Bcl-2, and cleaved caspase-3 protein levels. ( E ) Western blot analysis of STAT3 and p-STAT3 protein levels. ( F ) Quantification of p-STAT3 protein levels. ( G ) Flow cytometry analysis of apoptosis in Ctrl, H 2 O 2 , H 2 O 2 + loganin, and H 2 O 2 + loganin + IL-6 groups. ( H ) Quantification of apoptosis rate. ( I ) Western blot analysis of apoptosis-related proteins Bcl-2, BAX, pro-caspase-3, and cleaved caspase-3 in Ctrl, H 2 O 2 , H 2 O 2 + loganin, and H 2 O 2 + loganin + IL-6 groups. ( J – L ) Quantification of BAX, Bcl-2, and cleaved caspase-3 protein levels, respectively. ( M ) Western blot analysis of STAT3 and p-STAT3 protein levels. ( N ) Quantification of p-STAT3 protein levels. Data are presented as mean ± SD ( n = 4 per group). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; ns, not significant. For panels A–F, H 2 O 2 was compared with Ctrl, H 2 O 2 + loganin was compared with H 2 O 2 , and the loganin group was compared with Ctrl. For panels G–N, H 2 O 2 was compared with Ctrl, H 2 O 2 + loganin was compared with H 2 O 2 , and H 2 O 2 + loganin + IL-6 was compared with H 2 O 2 + loganin.

    Journal: Scientific Reports

    Article Title: Loganin alleviates oxidative stress-induced apoptosis by modulating mitochondrial function and STAT3 signaling in DSS-induced colitis and H 2 O 2 -injured Caco-2 cells

    doi: 10.1038/s41598-026-55447-3

    Figure Lengend Snippet: Loganin inhibits apoptosis through the IL-6/STAT3/BCL2 signaling pathway in H 2 O 2 -induced epithelial cell injury. ( A ) Western blot analysis of apoptosis-related proteins Bcl-2, BAX, pro-caspase-3, and cleaved caspase-3. ( B – D ) Quantification of BAX, Bcl-2, and cleaved caspase-3 protein levels. ( E ) Western blot analysis of STAT3 and p-STAT3 protein levels. ( F ) Quantification of p-STAT3 protein levels. ( G ) Flow cytometry analysis of apoptosis in Ctrl, H 2 O 2 , H 2 O 2 + loganin, and H 2 O 2 + loganin + IL-6 groups. ( H ) Quantification of apoptosis rate. ( I ) Western blot analysis of apoptosis-related proteins Bcl-2, BAX, pro-caspase-3, and cleaved caspase-3 in Ctrl, H 2 O 2 , H 2 O 2 + loganin, and H 2 O 2 + loganin + IL-6 groups. ( J – L ) Quantification of BAX, Bcl-2, and cleaved caspase-3 protein levels, respectively. ( M ) Western blot analysis of STAT3 and p-STAT3 protein levels. ( N ) Quantification of p-STAT3 protein levels. Data are presented as mean ± SD ( n = 4 per group). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; ns, not significant. For panels A–F, H 2 O 2 was compared with Ctrl, H 2 O 2 + loganin was compared with H 2 O 2 , and the loganin group was compared with Ctrl. For panels G–N, H 2 O 2 was compared with Ctrl, H 2 O 2 + loganin was compared with H 2 O 2 , and H 2 O 2 + loganin + IL-6 was compared with H 2 O 2 + loganin.

    Article Snippet: To establish the oxidative stress model, Caco-2 cells were exposed to H 2 O 2 (100 μM) for 12 h. For interleukin-6 (IL-6) supplementation experiments, recombinant human IL-6 (MedChemExpress, HY-P7044) was added at 10 ng/mL for 24 h in the H 2 O 2 + loganin group before subsequent analyses, with reference to previous studies using IL-6 stimulation in Caco-2 cells .

    Techniques: Western Blot, Flow Cytometry